Hmm.. calcium channel depolarisation occurs as part of the decomposition process, typically around 20-30 minutes after death. [which is what causes cadaveric spasms sometimes.] But that's for humans, and is dependent upon environmental conditions... at a guess based on the lack of response I'd say your frog was dead for at least an hour though...
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dancing_serpent
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Date: 2014-06-27 03:56 pm (UTC)[Next on CSI:internet!]